Mutational Study in the Exon2 of Osteoprotegrin Gene in Osteoporotic Women in Chahar Mahal va Bakhtiari Province of Iran

Background: Osteoporosis is a multifactorial disease. Mutation in cystein-rich domain2 (CRD2) and domain3 (CRD3) of osteoprotegrin protein can prevent its interaction with receptor activator nuclear factor kappa beta (RANKL) ligand lead to osteoporosis. Objectives: This study aimed investigate the possible alteration exon2 gene encoding CRD2 CRD3 domains osteoporotic women Chahar Mahal va Bakhtiari Province Iran. Methods: The N-terminal region human osteoprotegerin (OPG) was aligned mice proteins using Clustal Omega multiple alignment tool. genomic DNA 72 extracted by commercial kit. OPG amplified PCR sequenced sequencing. Results: result showed dissimilarities among three species terms domains. results polymerase chain reaction (PCR) amplification sequencing indicated that sequence were intact women. Conclusion: Due binding possibility RANKL ligand, it concluded expression may have been different However, recommended further studies should be conducted order confirm this finding.